NGS reagents - from kits to custom

Methylation analysis unlocks epigenetic insight, providing you with valuable information on various gene expression alterations found within DNA. Yet, genome-wide DNA methylation analysis by next-generation sequencing (NGS) can be challenging. Therefore, it’s essential to have a streamlined and reliable targeted methylation sequencing (Methyl-Seq) workflow that can provide more insight on the methylation patterns that matter to you.

This application note describes an autism spectrum disorder study using a modified protocol with  Agilent SureSelect XT HS2 library prep and SureSelect XT Methyl-Seq target enrichment to examine methylation status in murine model organisms exposed to valproic acid.

You’ll learn:

• Details on a Methyl-Seq workflow that combines the SureSelect XT HS2 library prep and SureSelect XT Methyl-Seq overnight hybrid capture, followed by bisulfite conversion.
• Benefits of a custom panel design for the SureSelect Methyl workflow leading to reduced sequencing costs combined with higher sequencing depth.
• Advantages of combining two complementary methylation data analysis methods.

See how Agilent SureSelect Methyl-Seq solutions can empower your genomics research.