Traditional measurement of viral titers during vaccine development often involve cytopathic effect (CPE) quantification by plaque assays or tissue culture infectious dose (TCID50) assays, which are based on subjective visual interpretation of cell culture deterioration. These labor-intensive endpoint assays can only provide a snapshot of the infection process and the overall kinetics information is missing.
The xCELLigence Real-Time Cell Analysis technology, a unique, live cell assay that allows label-free and continuous tracking of CPE progression in virus-infected cells, provides a dramatically simplified workflow for diverse virology assays, including but not limited to:
Topics covered in this webinar:
xCELLigence: a new real-time and high-throughput tool for viral infectivity titration
The classical cell-culture methods, such as cell culture infectious dose 50% (CCID50) assays, are time-consuming, endpoint assays currently used during the development of a viral vaccine production process to measure viral infectious titers. However, they are not suitable for handling the large number of tests required for high-throughput and large-scale screening analyses. Impedance-based bio-sensing techniques used in real-time cell analysis (RTCA) to assess cell layer biological status in vitro, provide real-time data. In this talk, we will assess the RTCA virus-titration assay as an alternative method to the classical CCID50 assay during the development of viral vaccine product
Monitoring Virus Cytopathic Effects in Real Time: Diverse Applications Ranging from Vaccine Development and Drug Discovery to Quantification of Neutralizing Antibodies
When infected by a virus, host cells typically display phenotypic changes (such as swelling, detachment, and lysis) that are collectively referred to as a cytopathic effect (CPE). Although CPEs can be a useful tool for monitoring viral infection, traditional methods like the plaque assay attempt to quantify CPEs manually, and are therefore labor-intensive, subjective, and suffer from poor reproducibility. Each of these challenges is overcome by the xCELLigence real time cell analyzer. Using gold biosensors embedded within the bottom of microplate wells, the xCELLigence instrument continuously monitors virus-induced changes in host cell number, cell size, cell-substrate attachment strength, and cell-cell adhesion ( barrier function). The high sensitivity and reproducibility, simple workflow, and real-time kinetics of this assay make it a powerful tool for diverse virology applications.
For Research Use Only. Not for use in diagnostic procedures.