Analyze Thermal Melt Faster, Bring Your Drug to Market Sooner

See how easy it is to achieve both speed and accuracy

Request our new information kit, featuring best practice tips, a case study from Vetter Pharma, application notes, and more.

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Save time without compromising the accuracy of your thermal melt experiments

Does your lab rely on UV-Vis to analyze the thermal melt temperature of nucleic acids and other therapeutic biomolecules? If so, you know that these measurements typically take an hour or more to complete—adding significant extra time to your schedule.

But what if you could analyze thermal melt more quickly without sacrificing data accuracy? Our new information kit can show you how. It includes a best practice guide that will help you optimize your thermal melt experiments for both time and accuracy. You’ll learn how to:

Choose the correct cuvettes and clean them.
Optimize sample concentration.
Avoid sample evaporation/contamination.
Degas samples and more.

To see how much time you can save on thermal melt experiments using the latest UV-Vis technologies, try this calculator.

How much time can the Agilent Cary 3500 UV-Vis save your lab?

Temperature range

Start temperature
Select your starting temperature
- 0°C
- 10°C
- 20°C
- 30°C
- 40°C
- 50°C
- 60°C
- 70°C
- 80°C
- 90°C
- 100°C
End temperature
Select your stopping temperature
- 0°C
- 10°C
- 20°C
- 30°C
- 40°C
- 50°C
- 60°C
- 70°C
- 80°C
- 90°C
- 100°C

Temperature ramp rate

Typical operating conditions
Select your temperature rate
- 0.5°C per minute
- 1°C per minute
- 2°C per minute
Agilent Cary 3500 UV-Vis^*

40°C per minute

Time to results

Typical operating conditions

Agilent Cary 3500 UV-Vis

You can save with the Cary 3500 UV-Vis for your thermal melt.

^* The Cary 3500 UV-Vis can be set from 0.1°C per minute up to 40°C per minute without compromising result accuracy. Thermal melt experimental conditions are sample dependent and it is recommended to validate the method.

Real labs, real results

Reliable Measurements in Less Than 10 Minutes

Vetter Pharma is successfully using the Cary 3500 UV-Vis to optimize thermal melt measurements of oligonucleotide-based pharmaceuticals.

Read case study

Characterization of Protein Thermal Stability Using UV‑Vis Spectroscopy

Researchers at Australia’s La Trobe University are performing fast, simple characterization of protein thermal stability and melting temperature.

Read application note

A New UV-Vis Approach for Protein Investigation

Carlos Santos-Martin of La Trobe University explores the principles and advantages of measuring thermal melt using UV-Vis.

Watch video

Video: Speed and accuracy in your UV‑Vis thermal melt assays

See how the Cary 3500 UV-Vis fits seamlessly into a GMP workflow, delivering accurate results quickly while ensuring data integrity.

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Be confident that your thermal melt work is ready for a regulated environment

Does your lab need to comply with guidelines such as FDA 21 CFR Part 11, EU Annex 11, GAMP5, ISO/IEC 17025, and EPA 40 CFR Part 160? Cary UV Workstation software, configured with Agilent OpenLab, ensures that your data will be managed, protected, and securely stored.

The Cary UV Workstation features built-in smoothing and derivative calculations for easy and automated data analysis. Learn more.

New information kit: Achieve efficient, compliant thermal melt analysis using UV-Vis

Request your free information kit, which includes:

Best practice guide: Top Tips for Measuring Thermal Melt. Which cuvette should I use? What’s the best way to degas samples? This quick-reference guide shows you how to optimize experimental conditions for thermal melt measurements.
Application note: Fast Determination of Thermal Melt Temperature of Double Stranded Nucleic Acids by UV-Vis Spectroscopy. The Cary 3500 UV-Vis accurately measures thermal melt temperatures regardless of a slow or fast ramp rate.
Application note: Characterization of Protein Thermal Stability Using UV-Vis. The Agilent Cary 3500 UV-Vis with Peltier temperature-controlled multicell reproducibly determined the melting temperature of EcDsbA protein samples.

Request yours now