Sialic Acid Analysis

The Agilent AdvanceBio Sialic Acid Profiling and Quantitation Kit represents a sensitive, high-throughput approach to both sialic acid profiling and quantitation. Workflow completed in ~5 hours.

Watch video

Sialic acid profiling identification and quantitation you can trust

Sialic acid is critical in mediating the effectiveness of therapeutic glycoproteins. The presence (or absence) of sialic acid on the non-reducing terminal of N- or O-glycans can dramatically affect the pharmacokinetics of the protein, as well as its immunogenicity. Therefore, it is essential that sialic acid content on protein therapeutics be maintained, controlled and monitored at the highest possible level.

Agilent products let you streamline your sialic acid workflows, from sample prep to analysis. Reduce sample prep time to just 5 hours, label sialic acid residues with a rapid, two-step procedure and perform high-throughput analysis directly from 96-well plates.

AdvanceBio Sialic Acid Profiling and Quantitation Kit: Generate downstream LC-FLD or LC-MS data you can trust with this complete sample prep and workflow solution. Identify sialic acid species with greater accuracy using a qualitative sialic acid reference panel (SARP), and perform absolute quantitation of sialic acids with quantitative NANA (Neu5Ac) and NGNA (Neu5Gc) standards.
AdvanceBio Sialic Acid Quantitation Kit: Provides rapid and reliable quantitation of total sialic acid content in your samples using fluorescence or absorbance detection using a plate reader.
InfinityLab Poroshell 120 EC-C18 column: Separate and analyze DMB-labeled sialic acids using a rapid, 10-minute UHPLC method with FLD detection for quantitation and optional MS detection for mass analysis.

Explore all AdvanceBio Sialic Acid kits and reagents.

Learn about Agilent’s glycan analysis solutions.

Explore innovations to streamline your workflow with monoclonal antibody CQA analysis.

Back to Top

Application Note: An Improved Workflow for Profiling and Quantitation of Sialic Acids in Biotherapeutics

Learn how the AdvanceBio Sialic Acid profiling and quantitation kit’s DMB labeling workflow can decrease your sample preparation time using rituximab, etanercept, NISTmAb and cetuximab as examples.

Download

Poster: Rapid High-Throughput Profiling and Quantitation of Sialic Acids in Biotherapeutics

Find out how to use this automation-friendly, plate-based workflow for the release, labeling, and analysis of sialic acids from therapeutic glycoproteins.

Download

Back to Top

Webinar: Glycosylation and Sialic Acid Analysis of Biotherapeutic Glycoproteins

Explore the benefits of different fluorescent dyes for released N-glycan analysis, HILIC-based separation options with FLD and MS detection, and sialic acid quantitation, analysis and species profiling using LC, FLD and MS.

Watch

Webinar: Multi-Level Workflows for Quantitative N-Glycan and Sialic Acid Analysis of Biotherapeutics

Learn about our complete N-glycan quantitation workflow solutions using LC with FLD or MS for four levels of biotherapeutic analysis and new workflows for quantitation of total sialic acid.

Watch

Back to Top

High-throughput Solutions for Sialic Acid Sample Prep and Analysis

Sialic acid profiling identification and quantitation you can trust

Your Personal Data

Your Purchase Plan