Is your purification lab compromising speed to obtain reliable research data? Agilent InfinityLab Poroshell 120 preparative LC columns let you maximize resolution while improving sample throughput.
Packed with 4 µm superficially porous particles (SPP), these columns maintain performance at higher flow rates—improving your speed from analytical through prep. So, you can successfully complete your purification goals faster and increase lab productivity.
Switching to InfinityLab Poroshell 120 preparative LC columns can give you more than two times your return on investment (ROI). And, you don’t even have to sacrifice speed or sample loading capacity.
Want a quick look at the numbers? Download our flyer
Need the full story? Download our ROI white paper
Agilent InfinityLab Poroshell 120 columns are based on superficially porous particle technology, which features a solid silica core and a porous outer layer. Compared to totally porous particles of the same (or similar) size, Poroshell particles give you higher chromatographic efficiencies and enable fast, high-resolution separations.
InfinityLab Poroshell 120 preparative columns let you realize the benefits of SPP technology without a trade-off on loadability. In this example, as mass load increases, the peak on the competitive traditional prep column broadens faster than on the InfinityLab Poroshell 120. This allows for a nearly 50% increase in sample load before reaching the same peak width as the competitive column.
InfinityLab Poroshell 120 columns are available in three different particle dimensions in analytical scale, so you can choose the size that best fits your separation needs and is most compatitble with your LC systems. Now, with the new InfinityLab Poroshell 120 preparative columns you can seamlessly scale up from sub-2 μm analytical work to prep.
InfinityLab preparative LC columns offer excellent bed stability and lifetime using our proprietary packing process for predictable performance. You can count on them to be robust under the most demanding operating conditions.
| Instrument: | Agilent 1290 Infinity II autoscale preparative LC system |
| Sample: | Ashwagandha extract in 2:1 ethanol:water, 100 mg/mL |
| Mobile phase: | A: Water + 0.1% formic acid B: Acetonitrile + 0.1% formic acid |
| Injection volume: | 1 mL filtered extract |
| Column 1: | Agilent InfinityLab Poroshell 120 SB-C18 21.2 x 150 mm, 4 μm |
| Column 2: | Competitor C18 19 x 150 mm, 5 μm |
Agilent run conditions
| Flow rate: | 37.5 mL/min |
| Gradient: | 5 to 95% B in 10 min |
Competitor run conditions
| Flow rate: | 17 mL/min |
| Gradient: | 5 to 95% B in 18 min |
The Agilent InfinityLab Poroshell 120 SB-C18 column provides superior resolution and baseline separation with the adjacent impurities, and maintains high resolution even when pushed to a flow rate of 1.5 times higher than optimal. This enables 45% time savings with high resolution separations.
| Instrument: | Agilent 1290 Infinity II preparative LC system |
| Sample: | Sulfanilamide |
| Column 1: | Agilent InfinityLab Poroshell 120 SB-C18 21.2 x 150 mm, 4 μm |
| Column 2: | Competitor C18, 19 x 150 mm, 5 μm |
| Mobile phase: | Column 1: 50:50 A:B Column 2: 53:47 A:B |
| A: Acetonitrile + 0.1% formic acid B: Water + 0.1% formic acid |
|
| Flow rate: | Column 1: 37.5 mL/min Column 2: 30 mL/min |
| Injection volume: | 900 μL |
Comparison of an Agilent InfinityLab Poroshell 120 SB-C18 4 μm preparative LC column with a competitive traditional preparative LC column. Chromatographic conditions for the traditional column were adjusted to match the linear velocity and sample retention on the InfinityLab Poroshell 120 column. As mass load increases, the peak on the competitive column broadens faster than on the InfinityLab Poroshell 120. This allows for a nearly 50% increase in sample load on the InfinityLab Poroshell 120 column before it reaches the same peak width as the traditional preparative column, offering you sharper peaks at higher mass loads.
| Instrument: | Agilent 1260 Infinity II LC system |
| Analytical columns: | Agilent InfinityLab Poroshell 120 SB-C18 3.0 x 50 mm, 1.9 μm, 2.7 μm, 4 μm |
| Flow rate: | 0.5 mL/min |
| Injection volume: | 5 μL |
| Instrument: | Agilent 1290 Infinity II preparative LC system |
| Preparative column: | Agilent InfinityLab Poroshell 120 SB-C18, 21.2 x 50 mm, 4 μm |
| Flow rate: | 25 mL/min |
| Injection volume: | 250 μL |
Separation of sulfa drugs from analytical to preparative scale
| Column 1: | Agilent InfinityLab Poroshell 120 SB-C18 21.2 x 150 mm, 4 μm |
| Flow rate: | 40 mL/min |
| Mobile phase: | 60:40 Acetonitrile:Water |
Agilent InfinityLab Poroshell 120 preparative LC columns deliver robust and reproducible performance even after 1000 injections.
See how you can achieve 45% time savings and high resolution separations with InfinityLab Poroshell 120 preparative columns.
Join us as we review the basics of purification and explore new techniques for maximizing performance at higher flow rates in complex samples. We’ll also cover drug discovery applications and column comparisons.
Does your lab have a need for speed? See how superficially porous particle technology lets you run at higher flow rates from analytical to prep, so you can get to the finish line faster.
Read how superficially porous particle (SPP) preparative LC columns can drive discovery work using conventional analytical and preparative hardware. You’ll also gain insights into making SPP columns part of your purification workflow.