This application note demonstrates: (i) separation of large synthetic oligos from their failure sequences, and (ii) separation of incompletely thiolated sequences from phosphorothioate oligos using strong anion exchange chromatography. The data indicate the importance of high temperature and open pore morphology for efficient separation.
This application note demonstrates scale-up of an analytical purification method to preparative scale on PLRP-S reversed phase columns, which consist of highly porous poly(styrene/ divinylbenzene) particles that are noted for their extreme chemical and temperature stability. > 99% pure ssRNA could be obtained at high yield using the scaled-up process.
This application note demonstrates how chromatographic separation and mass spectral quality can be optimized by careful selection of the appropriate ion pairing agent. These analyses were carried out on an Agilent 1290 Infinity II LC system with UV detection by DAD and an Agilent 6530 LC/Q-TOF.
Oligo therapeutics, such as ASO, si-RNAs, and aptamers are emerging modalities of treatment. Their synthesis is complex, and purity and quality are critical. Agilent can help with a comprehensive portfolio of instruments, consumables, standards, media and services to support oligonucleotide discovery to manufacturing.