Are you facing purification and analytical challenges to obtain high quality synthetic oligos?
Agilent has an extensive portfolio in oligonucleotide purification with industry-leading capabilities in ion-exchange chromatography to help you face your challenges.
This application note demonstrates: (i) separation of large synthetic oligos from their failure sequences, and (ii) separation of incompletely thiolated sequences from phosphorothioate oligos using strong anion exchange chromatography. The data indicate the importance of high temperature and open pore morphology for efficient separation.
This application note demonstrates scale-up of an analytical purification method to preparative scale on PLRP-S reversed phase columns, which consist of highly porous poly(styrene/ divinylbenzene) particles that are noted for their extreme chemical and temperature stability. > 99% pure ssRNA could be obtained at high yield using the scaled-up process.
This application note demonstrates how chromatographic separation and mass spectral quality can be optimized by careful selection of the appropriate ion pairing agent. These analyses were carried out on an Agilent 1290 Infinity II LC system with UV detection by DAD and an Agilent 6530 LC/Q-TOF.
Oligo therapeutics, such as ASO, si-RNAs, and aptamers are emerging modalities of treatment. Their synthesis is complex, and purity and quality are critical. Agilent can help with a comprehensive portfolio of instruments, consumables, standards, media and services to support oligonucleotide discovery to manufacturing.
From routine analysis to cutting-edge research, the InfinityLab LC Series offers the broadest portfolio of HPLC and UHPLC solutions for any application and budget.
The portfolio offers you an affordable and entry-level high-quality system, ideal workhorse system for laboratory-scale LC purification, automated scale-up from analytical scouting to gram-level purification to automated sample injection and flexible access to analytical and preparative columns.
Go untargeted and see more while identifying unknowns using time-of-flight (TOF/Q-TOF) technology that delivers simultaneous accuracy, speed, and isotopic fidelity. The reliable family of Agilent LC/MS instruments provides a range of capability and performance to solve any LC/MS analysis challenge.
The LC columns that offer the robustness necessary at high pH for successful analysis of oligonucleotides.
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DE41786346
For Research Use Only. Not for use in diagnostic procedures.