High Flow Peptide Quant

High Flow Peptide Quantitation For The Clinical Laboratory

Agilent is here to guide you on your peptide quant journey.

Peptide quantitation is rapidly growing in clinical research as scientists are translating peptide biomarkers identified in basic research to clinical research

and utility. This quantitation approach measures unique peptides from the proteins that are expected to be present in clinical research samples based on prior

knowledge. That is, from literature, genomic and proteomic studies, or combined proteogenomic analysis.

As you develop peptide quantitation methods for your clinical research, we can offer expert advice at every step: sample preparation, liquid chromatography

(columns, consumables, and analytical methodologies for separations), peptide mass spectrometry using sensitive triple quadrupole instrumentation, and data

analysis using reliable software. In addition, with our technology you can operate your peptide quant method using high flow rates. Scroll down the page to learn more.

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AssayMAP Bravo Protein Sample Prep Platform

Large Capacity Cartridges now available!

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Sample Preparation

Reproducibility in sample preparation has always been critical in clinical research. Preparing peptide samples involves many timed steps and reagent additions that can impact analytical reproducibility. Automating this process reduces variability and provides error-free operation with sample tracking.

The Agilent AssayMAP Bravo platform efficiently prepares protein and peptide samples for LC/MS analysis. This platform offers a simplified user interface with laboratory-tested peptide and protein sample preparation protocols to meet your clinical research needs.

Liquid Chromatography

AdvanceBio Peptide Mapping columns

The chromatographic columns used for peptide separations typically have larger pores to accommodate the larger molecules and include chromatographic media that minimizes non-specific binding of analytes for improved recovery. Agilent AdvanceBio Peptide Mapping columns deliver consistent, exceptional performance for separating and characterizing peptides.

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1290 Infinity II LC System

After the samples have been prepared and are ready for analysis, the next step is developing an efficient chromatographic method for the target peptides. The Agilent 1290 Infinity II liquid chromatography system gives you the ability to perform demanding separations with exceptional reliability and robustness. The UHPLC capability of the Agilent 1290 Infinity II LC system offers a variety of flow rates and column choices so that you can optimize your chromatographic methods for target peptides in complex matrices such as biofluids.

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6495C Triple Quadrupole LC/MS

Delivers the highest sensitivity among all commercially available TQ LC/MS systems

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Mass Spectrometry

For peptide quantitation, sensitivity and a wide dynamic range can be critical because the protein concentrations in biofluids may span many orders of magnitude. The Agilent 6495 Triple Quadrupole LC/MS system incorporates iFunnel technology. When combined with the Agilent Jet Stream ion source, iFunnel technology dramatically increases ion sampling, which results in lower detection limits. The 6495 is the proven choice for quantitative applications requiring superior analytical sensitivity, reliability, and overall system robustness. When maintenance is needed, the newly incorporated gate valve enables higher uptime and increased productivity by allowing you to replace the capillary without having to vent the instrument.

Data Analysis

MassHunter

Agilent MassHunter Workstation software includes an acquisition module for instrument control as well as both quantitative and qualitative data analysis. Data processing can be automated with MassHunter Quantitative Analysis tools to rapidly provide peptide quantitation results. These tools enable you to customize the results criteria that you use and will highlight the results that warrant further review.

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Skyline

Agilent MassHunter software provides tight integration with Skyline software. Designed for proteomics researchers, Skyline software (from the MacCoss Group at the University of Washington) supports MRM, dynamic MRM (dMRM), and triggered MRM (tMRM) modes in MassHunter so you can acquire the desired quantifier and qualifier signals in increasingly complex samples.

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For Research Use Only. Not for use in diagnostic procedures.
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