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Patented modifications for sgRNAs

Chemistry Makes All the Difference

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Advantages of SureGuide sgRNA Chemistry

High levels of on-target editing are observed across multiple cell lines including K562 and HepG2 cells and in human primary cells such as C34+ HSPCs.

Explore how Agilent SureGuide Purified Chemically Synthesized CRISPR sgRNAs improve your CRISPR studies. In this flyer, discover why chemistry makes all the difference and how our sgRNAs are:

  •         Produced via fully customized de novo synthesis, including the tracrRNA sequence
  •         Manufactured under ISO 13485 standards using a proprietary chemistry and purification method for high quality and reproducibility
  •         Comprised of sequences 90 to 120 nt in length using easy-to-use CRISPR Design software
  •         Packaged in quantities from 100 mg to 20 mg (3 to 600 nmol)
  •         Available with up to 10 chemical modifications (M, MS, D, S)
Download the CRISPR flyer

How sgRNA Chemical Modifications Work

sgRNA Modification Research

Agilent research into internal chemical modifications of the single-guide RNA shows improvements to CRISPR-Cas specificity.

Learn about the modifications

Our 3xMS Modifications

In this Nature Biotechnology publication, learn how patented 5' and 3' 3xMS end-modifications enhance synthetic single guide RNA stability and improve genomic editing efficiency.1

Read the publication

Leading CRISPR Nucleic Acid Synthesis

Building on a history of producing high-fidelity oligonucleotides, Agilent delivers robust, extended-length sgRNAs for your gene engineering methods.

In this video, listen as Aparna Krishnan, Ph.D., R&D Director, Agilent Technologies, and Ronda Allen, R&D Associate VP, Agilent Technologies, describe the advantages and applications of long, chemically synthesized sgRNAs.

Watch the video

SureGuide sgRNA Resources

CRISPR Ordering Tool

Easily configure your customized Agilent SureGuide Chemically Synthesized sgRNA sequences with Agilent CRISPR ordering tool.

Configure sgRNAs

Product Overview

The SureGuide sgRNA Product Overview details optimal use of sgRNAs for your CRISPR editing methods. Read the user manual to learn handling, quantifying, transfection, and more. 

Get the manual

For Research Use Only. Not for use in diagnostic procedures.

DE or RA#

  1. Hendel, A.; Bak, R. O.; Clark, J. T.; Kennedy, A. B.; Ryan, D. E.; Roy, S.; Steinfeld, I.; Lunstad, B. D.; Kaiser, R. J.; Wilkens, A. B.; Bacchetta, R.; Tsalenko, A.; Dellinger, D.; Bruhn, L.; Porteus, M. H. Chemically Modified Guide RNAs Enhance CRISPR-Cas Genome Editing in Human Primary Cells. Nat. Biotechnol. 2015, 33 (9), 985–989. https://doi.org/10.1038/nbt.3290.
  2. Daniel E. Ryan, David Taussig, Israel Steinfeld, Smruti M. Phadnis, Benjamin D. Lunstad, Madhurima Singh, Xuan Vuong, Kenji D. Okochi, Ryan McCaffrey, Magdalena Olesiak, Subhadeep Roy, Chong Wing Yung, Bo Curry, Jeffrey R. Sampson, Laurakay Bruhn, and Douglas J. Dellinger. Improving CRISPR–Cas specificity with chemical modifications in single-guide RNAs. Nucleic Acids Research, Vol. 46, No. 2 (2018); https://doi:10.1093/nar/gkx1199

For Research Use Only. Not for use in diagnostic procedures.

DE or RA#