With our on-demand webinars, you'll gain knowledge from industry experts on different topics in NGS sample QC, while learning how Agilent solutions are used for accurate and reliable data, and increased lab efficiency. Featuring in-depth examples and detailed explanations, you'll find the information you need to strengthen your research.
This webinar will highlight the importance of the Femto Pulse system in GeneCore workflows and present data that emphasize the significance of sample QC for project success. Long-read sequencing (LRS) has gained popularity in the research community due to improvements in the overall stability and quality of generated data. However, the value of LRS data is critically dependent on the quality of the input material used for library preparation. At the European Molecular Biology Laboratory (EMBL), the Agilent Femto Pulse system is a fundamental part of the daily routines.
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In this webinar, we describe a method to accurately determine the size of PCR-free libraries by adding a short PCR amplification step prior to automated DNA fragment analysis. Whole genome sequencing (WGS) and panel-based targeted sequencing provide vital genetic information for various applications. The quality of sequencing data hinges on the input library, traditionally prepared using PCR amplification, which can introduce biases and errors.
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This webinar will provide an overview of the applications and key benefits of the Agilent Femto Pulse system particularly its use in PacBio long-read sequencing workflows. Assessing the quality of genomic DNA is of utmost importance in NGS workflows. Recently, there has been a great interest in long-read sequencing, as it offers researchers the capability to analyze genomes more comprehensively and accurately.
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RNA sequencing unlocks the discovery of novel candidate biomarkers for diagnostic and therapeutic applications, but reproducibility is a key challenge in biomarker discovery programs. Integrating multifaceted quality control (QC) steps can improve design decisions and enhance the confidence and reliability of results. Learn how a comprehensive QC framework using the Agilent TapeStation system supports a disease-agnostic biomarker discovery program.
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Learn about optimization and quality control methodologies for preparation of bulk assay and single-cell ATAC sequencing libraries. Advancements in sequencing techniques have allowed for detailed exploration of chromatin accessibility, but the efficiency and quality of library preparation remain crucial to achieving reliable outcomes.
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Discover why accurate tumor-informed minimal residual disease detection (MRD) by NGS testing is highly dependent on maximum cfDNA recovery from plasma. For liquid biopsy applications, high recovery of total cfDNA is essential for detection of ctDNA. Due to requisite low limits of detection, accurate tumor-informed minimal residual disease detection (MRD) by NGS testing is highly dependent upon maximum recovery of cfDNA from plasma.
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This webinar addresses two topics: how to determine sample molarity to predict long-read sequencing read lengths and how to detect adapter dimers. Part one discusses how the Agilent Femto Pulse system and its data analysis software can be used to determine sample molarity to predict long-read sequencing read lengths. Part two explains how adapter dimers in a NGS library can have detrimental effects on downstream sequencing results.
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See how the European Reference Genome Atlas (ERGA) initiative has generated high-quality reference genomes for 95 species across 35
countries. The ERGA initiative, a pan-European consortium of over 500 members pursues the generation of high-quality reference genome assemblies of European species. To demonstrate the feasibility of this continent-wide collaboration, a pilot project aims to generate at least one high-quality reference genome for each European country.
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This webinar discusses the connection
between key sample quality control metrics and downstream sequencing results for high-quality NGS data production. Rigorous sample and library quality QC is essential to produce high-quality data in in NGS workflows. Dr. Maus, a bioinformatician at the NGS Core Facility of the German Cancer Research Center (DKFZ) shares his experience on the dependencies between the quality of samples, libraries, and its impact on NGS results.
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This webinar provides a comparison of
different methods to isolate high molecular weight (HMW) DNA from bacteria for downstream Nanopore sequencing. Nanopore long-read sequencing enables the resolution of repetitive regions and the assembly of closed bacterial genomes. The German NGS Competence Center in Tübingen tested different DNA isolation kits mostly dedicated for HMW DNA isolation and performed sample QC using the Agilent Femto Pulse system.
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This two-part webinar illustrates the effect
of daisy chains in NGS library preparations and how to determine the optimal NGS library read length. Part 1 discusses how to optimizing the amplification cycle number helps minimize daisy chain artifacts. Part 2 explains how sample QC enables researchers to confirm or adjust libraries for optimal insert length prior to NGS, helping to decrease background noise and improve quality scores of the sequencing run.
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This webinar teaches you the major points that need to be considered when scaling up sample throughput of the entire NGS workflow. Major points that need to be considered when increasing sample throughput of the entire NGS workflow are discussed. Attendees will understand how to scale-up with automation while maintaining data quality and improve consistency of results.
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Learn about a simplified process for automated NGS sample preparation, including steps for sample QC, target enrichment, and library preparation. In this webinar you can learn about a simplified sequencing workflow that enables an optimized, hands-free process for automated sample preparation using the Agilent Magnis NGS Prep system, SureSelect target enrichment system and Agilent QC systems.
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For Research Use Only. Not for use in diagnostic procedures.
PR7001-1937