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Application Compendium

Widefield Microscopy as a Screening Tool for Three- Dimensional Cell Culture

Page Title | Agilent

The goal of this application compendium is to introduce various imaging assays performed with the Agilent BioTek high-content imaging instruments and image analysis software to characterize phenotypic changes in cancer spheroids in response to different compound treatments.

We also propose optimized spheroid cell culture protocols for common spheroid cell culture models, such as low-adhesion U-bottom 96- and 384-well plates, Elplasia (microwell plates), and hanging drop method for three common cancer cell lines. Additionally, we introduce an improved workflow for staining that reduces assay time and minimizes variability by recommending suitable reagents and liquid handling systems.

In this Application Compendium, you’ll get valuable information about 3D Imaging Applications for Morphological and Phenotypic Characterization of 3D Cancer Spheroids such as:

  • Brightfield and fluorescence imaging using 3D PrimeSurface ultra-low attachment microplates
  • Utilizing automated imaging and advanced 3D cell culture techniques to quantify apoptosis activity
  • 3D Spheroid-based tumor invasion assay

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