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Application Compendium
 

Live Cell Proliferation and Viability

Live Cell Proliferation and Viability App Guide | Agilent

Counting the number of viable cells in a sample is a key step in many biological assays from normalization to measuring the effects of drugs on cell proliferation. Routine maintenance of cell culture also involves monitoring cell growth and counts for consistent cell seeding densities for downstream experiments. For live cell assays where proliferation is a key indicator of effectiveness, a non-destructive or label-free method of counting cells is desirable. Alternatively, destructive measures can be used, the most straightforward of which is to harvest cells and use a counting chamber for quantification

In this Application Compendium, you’ll get valuable information on applications such as:

  • Kinetic proliferation assay using label-free cell counting
  • Monitoring pH in long-term proliferation assays
  • Fluorescent markers in proliferation assays
  • Utilizing automated, multiparametric methods to quantify kinetic cell death analyses
  • Monitoring Saccharomyces cerevisiea growth with brightfield microscopy in real time

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