Counting the number of viable cells in a sample is a key step in many biological assays from normalization to measuring the effects of drugs on cell proliferation. Routine maintenance of cell culture also involves monitoring cell growth and counts for consistent cell seeding densities for downstream experiments. For live cell assays where proliferation is a key indicator of effectiveness, a non-destructive or label-free method of counting cells is desirable. Alternatively, destructive measures can be used, the most straightforward of which is to harvest cells and use a counting chamber for quantification
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