Application Compendium - Kinetic Fluorescence Live Cell Assays

The combination of kinetic live imaging with conventional fluorescence microscopy techniques has become an integral part of cellular analysis methodologies. Fluorescence imaging relies on illumination of fluorescent molecules with a defined wavelength of light ideally near the peak of the fluorophore excitation spectrum, and detection of light emitted at a longer wavelength. Commonly used live cell fluorescent molecules, such as fluorescent probes or dyes, and fluorescently labeled proteins have provided a range of tools to investigate virtually any cellular process of interest.

In this Application Compendium, you’ll get valuable information about Kinetic Fluorescence Live Cell Assays including:

Detecting cell cycle phase through incorporation of cell cycle/fluorescent protein fusions
Kinetic live cell imaging of apoptosis and necrosis
Characterizing calcium mobilization using kinetic live cell imaging
2D Scratch wound healing analysis of a co-cultured cell model
And much more...

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