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Becoming A Better Chromatographer

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Becoming A Better Chromatographer | Agilent

Golnar Javadi

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Dealing With Polar Analytes – What Are the Options?

25 February 2026 | 11 a.m. EST

Separation of highly polar compounds such as pharmaceuticals, metabolites, and biomarkers can be challenging using traditional reversed-phase methods on regular C18 columns. In this webinar, we will discuss available alternatives such as:

  • Ion-pairing reversed-phase chromatography.
  • Use of polar-bonded phases or polar-modified columns.
  • Alternative modes of separation, such as HILIC and Ligand Exchange chromatography.

Mohit Patel

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Advanced RP-HPLC Techniques for Peptide Analysis: From Column Chemistry to Mobile Phase Modifiers

19 March 2026 | 11 a.m. EST

Reversed-phase high-performance liquid chromatography (RP-HPLC) is a cornerstone technique for peptide analysis due to its exceptional resolving power. In this webinar, we will explore how to optimize RP-HPLC parameters for effective separation of peptides derived from protein digests and analysis of PTMs analysis. Key topics include the role of stationary phase chemistry in influencing selectivity, and how column physical characteristics affect separation performance. We will also examine how mobile phase modifiers—such as trifluoroacetic acid (TFA), formic acid, and pH—impact chromatographic outcomes and compatibility with mass spectrometry (MS). Attendees will gain practical insights into method development strategies that enhance both resolution and detection sensitivity.

Mark Powell

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Getting the Most from Your Agilent LCMS System

21 April 2026 | 11 a.m. EST

As demand for high lab productivity continues to increase, it is essential to ensure your LCMS system is ready to perform every day. In this webinar, we will discuss best practices and preventative maintenance tips to help maximize your instrument’s uptime. We will also take a quick look at column choices to help you get the most from your Agilent LCMS system.

On Demand Webinars

What’s Really Happening in Your Column: How Secondary Interactions Happen and What We Can Do about Them

On Demand | Anne Blackwell, Ph.D

Secondary interactions in HPLC are unintended interactions between the sample and any component of the flow path. They can lead to poor peak shape, poor resolution, and low sample recovery – and make it hard to interpret your chromatogram! An ideal separation is based purely on the primary separation mechanism, whether that is hydrophobicity in reversed-phase or size in size-exclusion chromatography. In reality, secondary interactions can happen in any kind of separation. In this webinar, we will discuss how they happen and how we can mitigate them, with tools such as mobile phase modifiers or inert LC column technology.  

HPLC Holiday Special: Proper Downtime Maintenance for Your Instrument

On Demand | Melissa Goodlad

The holiday season often means extra downtime for overworked scientists. Infrequent use or improper shutdown for your HPLC instrument can lead to blockages, biological growth, and decreased performance. Before you head out for the holidays, there are proactive steps you should consider for maintaining and servicing your HPLC. In this webinar we will cover how to properly shut down your instrumentation, so that it is ready to run as soon as the lab starts up again. We will also cover how to restart and what to do if your instrument was improperly stored. While the focus will be on Agilent equipment, we will cover many best practices across the analytical industry. With proper care, both you and your HPLC can return to work refreshed and ready for action.

Best Practices for HPLC Column Care and Maintenance

On Demand | Jean Lane

Proper care of your HPLC column is essential for consistent, high-quality results. In this webinar, we will cover best practices to maintain your column in optimal condition. Topics include proper connection techniques, mobile phase considerations, column equilibration, cleaning procedures, and correct storage methods. We will also address some common column related issues and offer troubleshooting strategies to help resolve them.  Whether you're working with Reverse Phase, HILIC, SEC, or IEX columns, being aware of your column’s technical specifications and applying careful handling can significantly extend the column’s performance and lifetime and have a lasting impact on your chromatography.

Sample Preparation for Food Safety Analysis

On Demand | Golnar Javadi

Effective sample preparation for food safety analysis should efficiently and selectively remove interferences to ensure accurate and consistent data. Additionally, it should aim to process as many samples as possible at minimal cost.

Preparing samples for food safety analysis can often be cumbersome, but it doesn't have to be. In this webinar, we'll explore straightforward passthrough options for food sample cleanup.  We'll provide examples of simple, innovative sample preparation solutions for analyzing mycotoxins, PFAS, pesticides, veterinary drugs, and PAHs across various food samples.

Benefits of Customizing LC systems for Bioanalysis and PFAS Analysis

On Demand | Mohit Patel

The use of extremely corrosive buffer salts in conjunction with extremely shallow gradients for the best separation makes Biochromatography a challenging application for applied liquid chromatography systems. Successful chromatography necessitates an iron-free flow channel, consistent gradient formation, and the capability to utilize either binary or quaternary pumping systems.

Discover how to make changes to the LC system to increase its biocompatibility. Discussion will include biocompatible flow channel for molecules that are prone to metal-induced artifacts. Additionally, we will discuss strategies for lowering PFAS backgrounds from LC instruments. In particular, PFC-free LC conversion kits and associated substitutes for all crucial LC system components made of materials containing organic fluorine compounds will be covered.

DE-001860

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Golnar Javadi

Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.

Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.

Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.

Mohit Patel

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.

Mark Powell

Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.