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Becoming A Better Chromatographer

Webinar Series

Becoming A Better Chromatographer | Agilent

Emily Parry, Ph.D.

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Navigating PFAS Complexity: Addressing the Unique Chromatographic Challenges of Ultra‑Short‑Chain PFAS

27 May 2026 | 11 a.m. EST

PFAS, often referred to as “forever chemicals,” are a diverse class of anthropogenic compounds that have become a major public health concern. Their wide range of structures and frequent presence in laboratory materials create unique analytical challenges. These challenges are especially pronounced for ultra‑short‑chain PFAS, whose high polarity, limited retention on conventional reversed‑phase columns, and tendency to coelute with matrix components make them particularly difficult to separate and quantify. In this webinar, we will discuss key considerations for selecting an LC separation technique and choosing appropriate consumables—along with strategies to address the chromatographic complexities of ultra‑short‑chain PFAS—to help streamline PFAS analysis.

Golnar Javadi

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Preparing for LC Analysis: What to Consider Ahead of Time

24 June 2026 | 11 a.m. EST

Whether you’re starting a new method or running a routine one, having a checklist to review before starting can help avoid chromatography problems.

In this webinar, we will discuss:

  • Sample characteristics (target analytes, matrix, etc.) and how to prepare the sample for LC analysis
  • Which supplies to use to get the best results possible
  • How to select the appropriate column, equilibrate it, benchmark it with your system, and how to take care of it
  • Getting to know your LC system, the importance of maintenance and having a “housekeeping” list.

Mark Powell

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Column Chemistry Compass: Navigating the Agilent HPLC and UHPLC Column Landscape

28 July 2026 | 11 a.m. EST

Choosing the right HPLC column can be challenging given the wide range of stationary phases, particle technologies, and column dimensions available today. Whether you’re aiming to accelerate your separations, extend the performance of an older LC system, or unlock the full capability of a modern Agilent UHPLC platform, selecting the optimal column is key to achieving reliable, reproducible results.

In this webinar, we’ll explore how Agilent’s column technologies—from traditional fully porous ZORBAX materials to advanced superficially porous InfinityLab Poroshell particles—can be strategically matched to your instrument and your analytical goals. We’ll discuss how particle size and column dimensions influence efficiency and back pressure, helping you balance resolution, speed, or instrument compatibility.

Beyond standard C18 phases, we’ll highlight a range of Agilent bonded phase chemistries designed to enhance selectivity and help you make informed choices for your next HPLC or UHPLC separation.

Jean Lane

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Why Your Column Isn’t Always the Culprit: Smarter HPLC Troubleshooting

12 August 2026 | 11 a.m. EST

Unexpected changes to our chromatography often lead us to suspect the column first.   But, in many cases, the true source of the problem is somewhere else in the system.  In this webinar, we’ll explore some of the common chromatography issues we most often hear about as technical support specialists. Whether you’re dealing with shifts in retention time, pressure fluctuations, unexpected peaks, ghost peaks, missing peaks, or your peak shapes just don’t look right, we’ll walk through practical steps for diagnosing and resolving the problem. Having an understanding for where issues typically occur can help us to quickly identify the problem, implement the right corrective action, and prevent the problem from recurring in the future.

Mohit Patel

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Comprehensive SEC Solutions: Performance and Application Insights Across Agilent’s SEC Columns

17 September 2026 | 11 a.m. EST

Size exclusion chromatography (SEC) is an essential technique for separating and characterizing biomolecules and polymers based on hydrodynamic size, enabling applications such as aggregate analysis of therapeutic proteins, assessment of viral vectors and nucleic acid therapeutics, and molecular‑weight profiling of synthetic polymers.

In this webinar, we will present a streamlined SEC workflow using Agilent LC platforms alongside Agilent’s full portfolio of SEC column solutions. The session will highlight performance characteristics, application fit, and best-practice method setup for multiple Agilent SEC columns—including our most advanced column technologies designed for high efficiency, minimal secondary interactions, and dependable robustness across complex sample types. Attendees will gain practical guidance on selecting the right column for their application, optimizing isocratic SEC conditions, and achieving reproducible, high-resolution separations in both R&D and QC environments. 

Melissa Goodlad

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Stocked for Success: Resources and Supplies to Minimize HPLC Downtime

8 October 2026 | 11 a.m. EST

If your goal is to maximize instrument uptime, then maintaining a well-stocked lab is your first step. Preventive maintenance is the key to minimizing instrument downtime. In this webinar, we will discuss which tools, supplies, and resources you should have on hand to maintain your instrument. Even with the best maintenance, sometimes instruments and methods need troubleshooting. This webinar will also discuss which resources and supplies can simplify your troubleshooting.

On Demand Webinars

What’s Really Happening in Your Column: How Secondary Interactions Happen and What We Can Do about Them

On Demand | Anne Blackwell, Ph.D

Secondary interactions in HPLC are unintended interactions between the sample and any component of the flow path. They can lead to poor peak shape, poor resolution, and low sample recovery – and make it hard to interpret your chromatogram! An ideal separation is based purely on the primary separation mechanism, whether that is hydrophobicity in reversed-phase or size in size-exclusion chromatography. In reality, secondary interactions can happen in any kind of separation. In this webinar, we will discuss how they happen and how we can mitigate them, with tools such as mobile phase modifiers or inert LC column technology.  

HPLC Holiday Special: Proper Downtime Maintenance for Your Instrument

On Demand | Melissa Goodlad

The holiday season often means extra downtime for overworked scientists. Infrequent use or improper shutdown for your HPLC instrument can lead to blockages, biological growth, and decreased performance. Before you head out for the holidays, there are proactive steps you should consider for maintaining and servicing your HPLC. In this webinar we will cover how to properly shut down your instrumentation, so that it is ready to run as soon as the lab starts up again. We will also cover how to restart and what to do if your instrument was improperly stored. While the focus will be on Agilent equipment, we will cover many best practices across the analytical industry. With proper care, both you and your HPLC can return to work refreshed and ready for action.

Best Practices for HPLC Column Care and Maintenance

On Demand | Jean Lane

Proper care of your HPLC column is essential for consistent, high-quality results. In this webinar, we will cover best practices to maintain your column in optimal condition. Topics include proper connection techniques, mobile phase considerations, column equilibration, cleaning procedures, and correct storage methods. We will also address some common column related issues and offer troubleshooting strategies to help resolve them.  Whether you're working with Reverse Phase, HILIC, SEC, or IEX columns, being aware of your column’s technical specifications and applying careful handling can significantly extend the column’s performance and lifetime and have a lasting impact on your chromatography.

Sample Preparation for Food Safety Analysis

On Demand | Golnar Javadi

Effective sample preparation for food safety analysis should efficiently and selectively remove interferences to ensure accurate and consistent data. Additionally, it should aim to process as many samples as possible at minimal cost.

Preparing samples for food safety analysis can often be cumbersome, but it doesn't have to be. In this webinar, we'll explore straightforward passthrough options for food sample cleanup.  We'll provide examples of simple, innovative sample preparation solutions for analyzing mycotoxins, PFAS, pesticides, veterinary drugs, and PAHs across various food samples.

Benefits of Customizing LC systems for Bioanalysis and PFAS Analysis

On Demand | Mohit Patel

The use of extremely corrosive buffer salts in conjunction with extremely shallow gradients for the best separation makes Biochromatography a challenging application for applied liquid chromatography systems. Successful chromatography necessitates an iron-free flow channel, consistent gradient formation, and the capability to utilize either binary or quaternary pumping systems.

Discover how to make changes to the LC system to increase its biocompatibility. Discussion will include biocompatible flow channel for molecules that are prone to metal-induced artifacts. Additionally, we will discuss strategies for lowering PFAS backgrounds from LC instruments. In particular, PFC-free LC conversion kits and associated substitutes for all crucial LC system components made of materials containing organic fluorine compounds will be covered.

Dealing With Polar Analytes – What Are the Options?

On Demand | Golnar Javadi

Separation of highly polar compounds such as pharmaceuticals, metabolites, and biomarkers can be challenging using traditional reversed-phase methods on regular C18 columns. In this webinar, we will discuss available alternatives such as:

Ion-pairing reversed-phase chromatography.
Use of polar-bonded phases or polar-modified columns.
Alternative modes of separation, such as HILIC and Ligand Exchange chromatography.

Advanced RP-HPLC Techniques for Peptide Analysis: From Column Chemistry to Mobile Phase Modifiers

On Demand | Mohit Patel

Reversed-phase high-performance liquid chromatography (RP-HPLC) is a cornerstone technique for peptide analysis due to its exceptional resolving power. In this webinar, we will explore how to optimize RP-HPLC parameters for effective separation of peptides derived from protein digests and analysis of PTMs analysis. Key topics include the role of stationary phase chemistry in influencing selectivity, and how column physical characteristics affect separation performance. We will also examine how mobile phase modifiers—such as trifluoroacetic acid (TFA), formic acid, and pH—impact chromatographic outcomes and compatibility with mass spectrometry (MS). Attendees will gain practical insights into method development strategies that enhance both resolution and detection sensitivity.

Getting the Most from Your Agilent LCMS System

On Demand | Mark Powell

As demand for high lab productivity continues to increase, it is essential to ensure your LCMS system is ready to perform every day. In this webinar, we will discuss best practices and preventative maintenance tips to help maximize your instrument’s uptime. We will also take a quick look at column choices to help you get the most from your Agilent LCMS system.

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Golnar Javadi

Golnar Javadi is an applications engineer providing online applications assistance and technical support for LC columns and supplies, Sample Preparation products, and Spectroscopy supplies.

Before joining Varian and then Agilent, Golnar worked in the pharmaceutical and nutraceutical industries on method development and quality control. She also taught college level chemistry courses for a few years.

Golnar then joined Varian as a technical support specialist, providing customers with method development assistance and technical support on Sample Preparation (SPE, SLE, and Filtration), HPLC, GC, ToxiLab and DAT. After the Varian-Agilent merger, Golnar joined Agilent Technologies as an Applications Engineer. She provides technical support to customers on Sample Preparation products, Liquid Chromatography columns, and Spectroscopy supplies.

Mark Powell

Mark Powell is an Applications Engineer located at Agilent’s Little Falls site in Wilmington, DE. Before joining Agilent in 2011, Mark worked in the pharmaceutical industry synthesizing, purifying and analyzing drug candidates. He provides applications assistance and technical support for Agilent’s HPLC columns and consumables.

Emily Parry, Ph.D.

Emily Parry holds a Ph.D. in Environmental Chemistry from the University of California, Davis, where she studied how wastewater treatment transforms environmental contaminants. After completing her degree, she began her career conducting research at the Environmental Protection Agency (EPA) and the Public Health Institute. In 2018, she joined Agilent Technologies as an application scientist focused on small‑molecule analysis using LC‑QTOF and LC‑TQ, specializing in environmental applications. In 2026, she transitioned to Agilent’s LC columns group, where she supports customers and applications in small‑molecule separations. Over the years, she has developed deep expertise in analytical methods for measuring small molecules across diverse sample types.

Melissa Goodlad

Melissa Goodlad is an Application Engineer providing applications assistance and technical support for Agilent’s HPLC columns and consumables. Before joining Agilent, Melissa was completing her PhD in Physical Chemistry at UC Merced studying nanoparticle self-assemblies.

Jean Lane

Jean has been employed with Agilent since Nov 2010 in technical and applications support and has a primary role supporting Agilent’s full line of LC columns and consumables, which also includes support for Agilent’s GPC/SEC product line. She came to Agilent thru acquisition of Varian and the former Polymer Laboratories. Jean has 20 plus years of technical and applications support for both HPLC and GPC consumable products. In addition to practical chromatography experience in the laboratory, she has had many, many years in her technical role involving customer support providing assistance and troubleshooting for customer chromatography issues.

Mohit Patel

Mohit Patel is part of the CSD Online AE Team. His primary focus is to support SMLC, Bio-Columns, and consumables.

Before joining Agilent, Mohit was an Assistant Professor of Chemistry at Temple University. Mohit developed his passion for science through his experiences as a faculty researcher and a mentor. Mohit actively taught, developed curriculum, and served as senior personnel, with a leadership position, on funded grants, mentored research projects, and co-coordinator of STEAM related outreach services.

Mohit has earned a Doctorate degree in Chemistry from Temple University in 2016.