Two new application notes show BAC fragment quality control under 3 hours

Bacterial artificial chromosome (BAC) libraries are versatile, well-established tools used for hosting entire genes. Accurate size analysis of BAC fragments is an important quality control assessment to verify that the entire gene of interest has been inserted appropriately. Typically, BAC fragment analysis has been performed through a 12+ hour process using pulsed-field gel electrophoresis (PFGE).

In two application notes, Agilent scientists demonstrate the performance of the new Agilent 165 kb BAC kit designed for use on the Agilent Femto Pulse system. When compared with conventional PFGE, they found that the system enabled automated quality control of BAC DNA fragments with high sensitivity, accuracy, and speed. The improved sizing precision ensures the proper size of gene insertions. Samples processed with the 165 kb BAC kit on the Femto Pulse system enabled analysis of both large and small BAC fragments in under three hours. These two application notes highlight:

• How the analysis of large and small fragments can be visualized in one run with accurate sizing from 75 to 165,000 bp
• How to achieve faster time to results with a reduced turn-around time from >12 hours to approximately 3 hours
• How to conserve sample by only using amounts in the picogram range
• How to routinely generate, reproducible results with automated fragment analysis

For Research Use Only. Not for use in diagnostic procedures.